Accurate quantitative phase imaging through telecentric digital holo- graphic microscopy
نویسندگان
چکیده
The use of non-telecentric imaging systems in quantitative phase digital holographic microscopy introduces strong inaccuracies. We show that even negligible errors in the radius and center of curvature of the remaining quadratic phase factor introduce big errors in the numerical phase measurements. The errors depend on the position of the object in the field-of-view. However, when a telecentric imaging system is utilized for the recording of the holograms, the hybrid imaging method shows shift-invariant behavior, and therefore accurate quantitative phase imaging can be performed.
منابع مشابه
Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy.
The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the microscope allows single-shot QPI in DHM by using telecentric imaging systems. Quan...
متن کاملAberration compensation for objective phase curvature in phase holographic microscopy: comment.
In a recent Letter by Seo et al. [Opt. Lett.37, 4976 (2012)], the numerical correction of the quadratic phase distortion introduced by the microscope objective in digital holographic microscopy (DHM) has been presented. In this comment, we would like to draw to the attention of the authors and the readers in general that this approach could not be the optimal solution for maintaining the accura...
متن کاملPhysical compensation of phase curvature in digital holographic microscopy by use of programmable liquid lens.
Quantitative phase measurements obtained with digital holographic microscopes are strongly dependent on the optical arrangement of the imaging system. The nontelecentric operation provides phase measurements affected by a parabolic phase factor and requires numerical postprocessing, which does not always remove all the perturbation. Accurate phase measurements are achieved by using the imaging ...
متن کاملDesign of angle-resolved illumination optics using nonimaging bi-telecentricity for 193 nm scatterfield microscopy.
Accurate optics-based dimensional measurements of features sized well-below the diffraction limit require a thorough understanding of the illumination within the optical column and of the three-dimensional scattered fields that contain the information required for quantitative metrology. Scatterfield microscopy can pair simulations with angle-resolved tool characterization to improve agreement ...
متن کاملDigital holographic microscopy with pure-optical spherical phase compensation.
Telecentric architecture is proposed for circumventing, by the pure-optical method, the residual parabolic phase distortion inherent to standard configuration of digital holographic microscopy. This optical circumvention produces several important advantages. One is that there is no need for computer compensation of the parabolic phase during the phase map recovering procedure. The other is tha...
متن کامل